SOPs

These Standard Operating Procedures (SOPs) were developed as part of a project funded by the International Pathogen Surveillance Network (IPSN) of the World Health Organization. The project was carried out in collaboration with the following partners: Université Évangélique en Afrique (Bukavu), Hôpital de Panzi, Utrecht University, and Université libre de Bruxelles (ULB).

Data Analysis SOPs

1.IPSN_RunNanoporeMinionSequencerDownload
2.IPSN_installMinKnowSoftwareDownload
3.IPSN_installEPI2MESoftwareDownload
4.IPSN_AnalysisNanoporeDataWithEpi2MEDownload
5.IPSN_AnalysiswithCGEWebToolsDownload
6.IPSN_PathogenWatch_OwncloudDownload

Sequencing in Mini-Lab

10.3.2 Subculture-Purity-SOP-V1Download
10.3.3- DNA Extraction from pure bacterial cultures-SOP-V1Download
10.3.4- DNA Quantification using Qubit-SOP-V1Download
10.3.5 Rapid sequencing gDNA -SOP-V1Download
10.3.6 Priming and loading a flow cell -SOP-V1Download
10.3.7 Washing a flow cell -SOP-V1Download